Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin

Nature. 1984 Dec 20-1985 Jan 2;312(5996):724-9. doi: 10.1038/312724a0.

Abstract

Human tumour necrosis factor has about 30% homology in its amino acid sequence with lymphotoxin, a lymphokine that has similar biological properties. Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics
  • Escherichia coli / genetics
  • Gene Expression Regulation
  • Glycoproteins / genetics*
  • Glycoproteins / therapeutic use
  • Humans
  • Lymphokines / genetics
  • Lymphotoxin-alpha / genetics
  • Mice
  • Protein Precursors / genetics
  • Sarcoma, Experimental / therapy
  • Tumor Necrosis Factor-alpha

Substances

  • Glycoproteins
  • Lymphokines
  • Lymphotoxin-alpha
  • Protein Precursors
  • Tumor Necrosis Factor-alpha
  • DNA

Associated data

  • GENBANK/X01394
  • GENBANK/X02159
  • GENBANK/X02910