The effects of sodium hyaluronate, chondroitin sulfate, and methylcellulose on the corneal endothelium and intraocular pressure

Am J Ophthalmol. 1983 Mar;95(3):332-41. doi: 10.1016/s0002-9394(14)78301-1.


Sodium hyaluronate (Healon), chondroitin sulfate, and methylcellulose have been used to protect the corneal endothelium from intraocular lens trauma. A study of the efficacy and toxicity of these compounds showed that 1% sodium hyaluronate, 0.4% methylcellulose, and 20% chondroitin sulfate were nontoxic to the corneal endothelium, but that 20% chondroitin sulfate caused a marked decrease in corneal thickness because of its hypertonicity. Anterior chamber injection of these viscous substances resulted in an increase in intraocular pressure. Within one to four hours the maximum intraocular pressure with 1% sodium hyaluronate was 67 +/- 4.1 mm Hg and that with 20% chondroitin sulfate was 55 +/- 3.5 mm Hg. The intraocular pressure did not increase to these high levels with 10% chondroitin sulfate or 0.4% methylcellulose or when the test substances were washed out of the anterior chamber. The corneal endothelium was protected from injury with 1% sodium hyaluronate and 20% chondroitin sulfate, but 10% chondroitin sulfate and 0.4% methylcellulose provided only minimal protection.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chondroitin / analogs & derivatives*
  • Chondroitin Sulfates / pharmacology*
  • Chondroitin Sulfates / therapeutic use
  • Cornea / drug effects*
  • Corneal Injuries
  • Endothelium / drug effects
  • Hyaluronic Acid / pharmacology*
  • Hyaluronic Acid / therapeutic use
  • Intraocular Pressure / drug effects*
  • Lenses, Intraocular / adverse effects
  • Methylcellulose / pharmacology*
  • Methylcellulose / therapeutic use
  • Rabbits


  • Hyaluronic Acid
  • Methylcellulose
  • Chondroitin
  • Chondroitin Sulfates