Detection of Brucella Antibodies of Different Immunoglobulin Classes in Cow Milk by Enzyme-Linked Immunosorbent Assay

Am J Vet Res. 1983 Feb;44(2):306-8.

Abstract

Enzyme-linked immunosorbent assays were conducted on milk of cows from which Brucella abortus was isolated and that of noninfected controls. Horseradish peroxidase-labeled rabbit antibovine immunoglobulins IgG, IgG1, and IgA were used as conjugates. A heat-killed whole-cell suspension of B abortus strain 19 was used as the antigen. Differences in antibody profiles were observed in milk of cows from which B abortus was isolated and in milk of noninfected cows. Antibody profiles were similar in milk of cows infected with B abortus and that of cows from which B abortus strain 19 was isolated.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / analysis*
  • Brucella abortus / immunology*
  • Brucellosis, Bovine / immunology*
  • Cattle
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Immunoglobulin A / analysis
  • Immunoglobulin G / analysis
  • Immunoglobulins / analysis*
  • Milk / immunology*

Substances

  • Antibodies, Bacterial
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulins