Structure and expression of a cloned cDNA for human interleukin-2

Nature. 1983 Mar 24-30;302(5906):305-10. doi: 10.1038/302305a0.

Abstract

A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which consists of 153 amino acids including a putative signal sequence. A biologically active polypeptide, characteristic of human IL-2, was produced when the cDNA was fused to a simian virus 40 promoter sequence and used to transfect cultured monkey COS cells.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression Regulation
  • Haplorhini
  • Humans
  • Interleukin-2 / genetics*
  • Operon
  • Plasmids

Substances

  • Interleukin-2
  • DNA

Associated data

  • GENBANK/J00264