Anti-Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage

J Immunol. 1983 Jul;131(1):212-6.


Anti-Leu-3a, anti-Leu-3b, OKT4, and anti-T4 murine monoclonal antibodies react with a membrane component expressed by mature peripheral blood helper T cells and certain thymocyte subsets. Using a variety of immunologic staining techniques, we have demonstrated the reactivity of these antibodies with other cell types. Normal and neoplastic cells of monocyte/macrophage lineage bear the Ia+/Leu-6-/Leu-3+ phenotype, whereas histiocytosis X cells bear the Ia+/Leu-6+/Leu-3+ phenotype. The Ia+/Leu-6- cells of malignant histiocytosis and the Ia+/Leu-6+ epidermal Langerhans cells were variably Leu-3+. Normal monocyte/macrophage reactivity with anti-Leu-3/T4 appears to be primarily intracytoplasmic, whereas on U937 monocyte tumor cells, marked membrane reactivity is also observed. These results strongly suggest that certain cells other than helper T cells and thymocytes can express and, at least in some cases, synthesize a component previously regarded as T-lineage specific.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / physiology*
  • Antigen-Antibody Reactions
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface / analysis
  • Cell Differentiation
  • Cell Line
  • Flow Cytometry
  • Humans
  • Immunoenzyme Techniques
  • Langerhans Cells / cytology
  • Langerhans Cells / immunology*
  • Macrophages / cytology
  • Macrophages / immunology*
  • Monocytes / cytology
  • Monocytes / immunology*


  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface