Derivation of Monoclonal Antibodies Against Brucella Abortus Antigens

Vet Immunol Immunopathol. 1983 Jul;4(5-6):603-14. doi: 10.1016/0165-2427(83)90068-5.

Abstract

In vivo immunization, fusion, antibody detection, and cryopreservation procedures for monoclonal antibody production against antigens of Brucella abortus are described. Splenocytes from BALB/c mice immunized with irradiated B. abortus S2308 were fused with Sp2/O-Ag14 myeloma cells and 61 hybridomas secreting anti-Brucella antibodies were cloned. Hybridoma antibody synthesis was detected effectively and most efficiently by enzyme-linked immunosorbent assays. Antibodies from clones of hybridoma A23 reacted with S19 and S2308 whole bacterial cells, while hybridoma B49 reacted primarily with alkali--treated lipopolysaccharides of S19, S1119.3 and S2308. Cryopreservation of clones had no major effect on antibody synthesis. The application of monoclonal anti-Brucella antibodies in the differential diagnosis of bovine brucellosis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibody Specificity
  • Antigens, Bacterial / immunology*
  • Brucella abortus / immunology*
  • Brucellosis, Bovine / diagnosis
  • Brucellosis, Bovine / immunology
  • Cattle
  • Diagnosis, Differential
  • Mice

Substances

  • Antibodies, Monoclonal
  • Antigens, Bacterial