Characterization of the human receptor for T-cell growth factor

Proc Natl Acad Sci U S A. 1983 Nov;80(22):6957-61. doi: 10.1073/pnas.80.22.6957.

Abstract

Anti-Tac monoclonal antibody has been identified as a putative antibody against the receptor for T-cell growth factor (TCGF). We now show that: (i) TCGF blocks 85% of 3H-labeled anti-Tac binding to phytohemagglutinin-activated lymphoblasts and (ii) both anti-Tac and anti-TCGF immunoprecipitate a protein band that appears to represent TCGF crosslinked to its receptor on HUT-102B2 cells. In HUT-102B2 cells, the TCGF receptor is a Mr 50,000 glycoprotein with internal disulfide bond(s) and a pI of 5.5-6.0, and it represents approximately equal to 0.05% of total cellular de novo protein synthesis. It contains a peptide of Mr 33,000 that is processed to a mature form that includes N-linked and O-linked sugars and sialic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Glycoside Hydrolases / pharmacology
  • Humans
  • Interleukin-2 / immunology*
  • Kinetics
  • Lymphoma / immunology
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
  • Molecular Weight
  • Neuraminidase / pharmacology
  • Receptors, Immunologic / immunology*
  • Receptors, Immunologic / isolation & purification
  • Receptors, Interleukin-2
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Tunicamycin / pharmacology

Substances

  • Interleukin-2
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Tunicamycin
  • Glycoside Hydrolases
  • Neuraminidase
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase