Mutagenicity evaluation of glutaraldehyde in a battery of in vitro bacterial and mammalian test systems

Food Chem Toxicol. 1983 Oct;21(5):621-9. doi: 10.1016/0278-6915(83)90150-3.


Glutaraldehyde was evaluated for genotoxicity using a battery of four in vitro test systems: the Salmonella/microsome assay, the Chinese hamster ovary cell/hypoxanthine-guanine phosphoribosyltransferase (CHO/HGPRT) gene mutation system, the sister-chromatid exchange test with Chinese hamster ovary cells, and measurements of unscheduled DNA synthesis in primary rat-hepatocyte cultures. No significant, dose-related increases in the various end-points were produced by glutaraldehyde in tests with or without the addition of a rat-liver metabolic activation system (S-9 mix) or with the cell-mediated activation of the hepatocyte test system. A range of concentrations which spanned cytotoxic to non-cytotoxic doses was evaluated in each test system and marked cytotoxicity was typically noted at micromolar concentrations. Within a range of biologically active concentrations, glutaraldehyde did not produce significant genotoxic effects with the assays and conditions used for these studies.

MeSH terms

  • Aldehydes / toxicity*
  • Animals
  • Biotransformation
  • Cell Line
  • Cell Survival / drug effects
  • Cricetinae
  • Cricetulus
  • DNA / biosynthesis
  • Female
  • Genes / drug effects
  • Glutaral / toxicity*
  • Hypoxanthine Phosphoribosyltransferase / genetics
  • Liver / metabolism
  • Male
  • Mutagenicity Tests / methods
  • Mutagens*
  • Ovary
  • Rats
  • Rats, Inbred Strains
  • Salmonella typhimurium / drug effects
  • Sister Chromatid Exchange / drug effects


  • Aldehydes
  • Mutagens
  • DNA
  • Hypoxanthine Phosphoribosyltransferase
  • Glutaral