Newly administered [3H]retinol is transferred from hepatocytes to stellate cells in liver for storage

Exp Cell Res. 1984 Jan;150(1):186-93. doi: 10.1016/0014-4827(84)90713-4.


We have recently shown that newly administered vitamin A (retinol) is initially taken up by the parenchymal cells of the liver, and subsequently (within 1-2 h) transferred to non-parenchymal liver cells (NPC) (Blomhoff et al., ref. [10]). In the present study we have separated the NPC by different methods to determine the cell type responsible for this uptake of [3H]retinol. When liver cells were prepared between 5 and 18 h after intraduodenal administration of [3H]retinol, the radioactive retinol was recovered mainly in the stellate cells. Other liver cells (i.e., hepatocytes, endothelial cells and Kupffer cells) contained only small amounts of [3H]retinol. Further, fluorescence microscopy studies indicated that stellate cells contain large quantities of retinol. Our results show that newly administered [3H]retinol, which is initially located in the hepatocytes, is transferred to the stellate cells and stored there.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Separation
  • Endothelium / cytology
  • Endothelium / metabolism
  • Enteral Nutrition
  • Kupffer Cells / metabolism
  • Liver / cytology
  • Liver / metabolism*
  • Microscopy, Fluorescence
  • Rats
  • Tissue Distribution
  • Vitamin A / administration & dosage
  • Vitamin A / metabolism*


  • Vitamin A