Relation between D-glucose and L- and D-alanine in the initiation of germination of Bacillus subtilis spore

Microbiol Immunol. 1984;28(2):197-207. doi: 10.1111/j.1348-0421.1984.tb00671.x.

Abstract

The rate of L-alanine-initiated germination of Bacillus subtilis spore was measured by both loss of heat resistance and loss of turbidity, and the effect of glucose on the germination response to a wide range of concentrations of the germinant was analyzed in the presence and absence of D-alanine, an inhibitor. Glucose stimulated L-alanine germination by means of a cooperative effect: glucose increased the affinity of L-alanine by about 3-fold and the rate of germination by about 1.3-fold. However, glucose had little effect on the binding affinity of D-alanine. The apparent binding constant of L-alanine to the spore, which was determined by the next measurable event in the trigger reaction, was 1.2 X 10(-5), that of D-alanine was 6 X 10(-6), and that of glucose was 5 X 10(-5). The relation between the binding site for glucose and those for L- and D-alanine on the spore is discussed. Effect of glucose analogs was also examined.

Publication types

  • Comparative Study

MeSH terms

  • Alanine / metabolism
  • Alanine / pharmacology*
  • Bacillus subtilis / drug effects
  • Bacillus subtilis / metabolism
  • Bacillus subtilis / physiology*
  • Binding Sites / drug effects
  • Carbohydrates / pharmacology
  • Dose-Response Relationship, Drug
  • Glucose / metabolism
  • Glucose / pharmacology*
  • Kinetics
  • Spores, Bacterial / drug effects
  • Spores, Bacterial / metabolism
  • Stereoisomerism
  • Time Factors

Substances

  • Carbohydrates
  • Glucose
  • Alanine