Cell surface proteins of Drosophila. II. A comparison of embryonic and ecdysone-induced proteins

Dev Biol. 1984 Jul;104(1):1-8. doi: 10.1016/0012-1606(84)90030-7.

Abstract

The cell-surface proteins of Drosophila embryos at gastrula and myoblast fusion stages were characterized by radioiodination and two-dimensional gel electrophoresis. Over 13% of the cell surface proteins detected in gastrula embryos were not found in myoblast fusion stage embryos or in Drosophila embryonic cell line EH34A3 cells. Nearly 18% of the cell-surface proteins detected in myoblast fusion stage embryos were evident only at that stage. Embryonic cell-surface proteins were compared with cell-surface proteins from untreated EH34A3 cells and EH34A3 cells treated with 20-hydroxyecdysone, which induces cell aggregation and the expression of "new" proteins at the cell surface (D. F. Woods and C. A. Poodry, 1983, Dev. Biol. 96, 23-31). Only one of the proteins induced by ecdysone in EH34A3 cells was detected in the NP-40 soluble fraction of radioiodinated cell lysates, even after fractionation by lectin affinity chromatography and immunoprecipitation to enrich for putative ecdysone induced proteins. However, extraction of the NP-40 insoluble pellet of embryo cells revealed one additional protein that was present both in myoblast fusion stage embryos and hormone-treated culture cells. It was concluded that except for these two proteins, the cell-surface proteins induced in cultured cell lines by treatment with 20-hydroxyecdysone are not present in significant amounts in gastrula or myoblast fusion stage embryos.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Antibody Complex
  • Cell Membrane / analysis
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / metabolism*
  • Ecdysone / pharmacology*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Gastrula / drug effects
  • Gastrula / metabolism
  • Immune Sera
  • Membrane Proteins / biosynthesis*

Substances

  • Antigen-Antibody Complex
  • Immune Sera
  • Membrane Proteins
  • Ecdysone