Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation

Science. 1984 Aug 17;225(4663):687-93. doi: 10.1126/science.6431612.

Abstract

Antisera to a synthetic c-myc peptide and to c-myc antigens synthesized from various portions of the human gene expressed in Escherichia coli were used in order to characterize the protein product of the human c-myc oncogene. Although the deduced molecular weight of the human c-myc protein is 49,000, these antisera precipitate a protein from human cells that migrates in sodium dodecyl sulfate-polyacrylamide gel as if its molecular weight were 65,000. In addition, the mouse c-myc protein, whether synthesized in cells or in a cell-free system directed by pure, synthetic messenger RNA, has analogous properties and is immunoprecipitated by the antiserum to the human c-myc protein. Similar proteins are immunoprecipitated from monkey, rat, hamster, and frog cells, suggesting evolutionary conservation of antigenic structure of the c-myc protein among vertebrates. In addition, and in a manner consistent with the behavior of its messenger RNA, the immunoprecipitable c-myc protein is sharply induced by the action of mitogens on resting human T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Neoplasm / immunology*
  • Base Sequence
  • Cell Division*
  • Chickens
  • Cricetinae
  • DNA, Neoplasm / genetics
  • DNA, Recombinant / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Haplorhini
  • Humans
  • Mice
  • Mitogens / pharmacology
  • Molecular Weight
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology*
  • Oncogenes*
  • RNA, Messenger / genetics
  • Rabbits
  • Rats

Substances

  • Antibodies, Neoplasm
  • DNA, Neoplasm
  • DNA, Recombinant
  • Mitogens
  • Neoplasm Proteins
  • RNA, Messenger