Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes

Cell. 1984 Aug;38(1):101-8. doi: 10.1016/0092-8674(84)90530-0.


Certain fluorescent dyes, previously reported to localize mitochondria, when used at higher concentrations also localize a continuous net-like structure in both living and glutaraldehyde-fixed cells. A similar reticular structure can be detected by phase-contrast microscopy and whole-mount electron microscopy in potassium permanganate-fixed cells as well. This structure is mostly tubular, with some patch-like areas, and is likely to be the endoplasmic reticulum (ER). The organization of the reticular structure is sensitive to colchicine and rotenone but not to cytochalasin B, taxol, monensin, the calcium ionophore A23187, 12-O-tetradecanoylphorbol 13-acetate, or hydrocortisone.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cell Line
  • Chick Embryo
  • Chlorocebus aethiops
  • Embryo, Mammalian
  • Endoplasmic Reticulum / ultrastructure*
  • Fibroblasts / cytology
  • Fluorescent Dyes
  • Glutaral
  • Kidney
  • Microscopy, Fluorescence
  • Microscopy, Phase-Contrast
  • Muscle, Smooth / cytology
  • Rats


  • Fluorescent Dyes
  • Glutaral