Transfection of the DNA polymerase-alpha gene

Science. 1984 Nov 16;226(4676):833-5. doi: 10.1126/science.6436977.


DNA polymerase-alpha is the major replicative DNA polymerase in animal cells. The gene coding for a mutant DNA polymerase-alpha was transferred from one cell to another by transfection of DNA from mutant cells. The DNA was isolated from a mutant hamster cell line resistant to aphidicolin, a specific inhibitor of DNA polymerase-alpha, and transferred into an aphidicolin-sensitive cell line. The resulting transfectants exhibited increased survival in the presence of aphidicolin and contained an aphidicolin-resistant DNA polymerase-alpha.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aphidicolin
  • Cell Line
  • Clone Cells
  • Cricetinae
  • Cricetulus / genetics
  • DNA Polymerase II / genetics*
  • Diterpenes / pharmacology
  • Escherichia coli / genetics
  • Humans
  • Mice
  • Mutation
  • Salmon / genetics
  • Transfection*


  • Diterpenes
  • Aphidicolin
  • DNA Polymerase II