Human interleukin-2 promotes proliferation of activated B cells via surface receptors similar to those of activated T cells

Nature. 1984 Dec 13-19;312(5995):641-3. doi: 10.1038/312641a0.

Abstract

Human interleukin-2 (IL-2) is a glycoprotein of relative molecular mass (Mr) 15,000, which is released by T lymphocytes on stimulation with antigen or mitogen and functions as a T-cell growth factor (TCGF) by inducing proliferation of activated T cells. It is generally accepted that resting or activated B cells do not respond directly to IL-2 but require for their proliferation other T-cell-derived lymphokines usually referred to as B-cell growth factors (BCGFs). Recently, however, a monoclonal antibody reacting with the IL-2 receptor molecules expressed by activated T cells (anti-Tac) was shown to react also with certain B tumour cells; in addition, murine B cells proliferate in response to pure human IL-2. We now show that recombinant IL-2, derived from Escherichia coli expressing the human gene, is able to promote strong proliferation of human B cells activated with protein-A-rich Staphylococcus aureus Cowans strain I. Moreover, we demonstrate that the anti-Tac antibody also reacts with S. aureus-activated normal B cells and inhibits sharply the proliferative response of such cells to IL-2. Finally, immunoprecipitation experiments reveal that anti-Tac defines similar molecules on activated T and B cells.

MeSH terms

  • Animals
  • Antigen-Antibody Complex
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • Cytotoxicity, Immunologic
  • Humans
  • Immune Sera
  • Interleukin-2 / immunology*
  • Interleukin-2 / pharmacology
  • Lymphocyte Activation*
  • Mice
  • Receptors, Immunologic / immunology*
  • Receptors, Interleukin-2
  • Spleen / immunology
  • Staphylococcus aureus / immunology
  • T-Lymphocytes / immunology*

Substances

  • Antigen-Antibody Complex
  • Immune Sera
  • Interleukin-2
  • Receptors, Immunologic
  • Receptors, Interleukin-2