Regulatory effects of insulin and experimental diabetes on neutral amino acid transport in the perfused rat exocrine pancreas. Kinetics of unidirectional L-serine influx and efflux at the basolateral plasma membrane

Biochim Biophys Acta. 1984 Dec 19;778(3):618-22. doi: 10.1016/0005-2736(84)90415-2.


Relatively little is known about the hormonal regulation of amino acid transport in the normal and diabetic exocrine pancreas. In this study unidirectional influx and tracer efflux of L-serine at the basolateral interface of the rat pancreatic epithelium was investigated in the perfused exocrine pancreas using a rapid (less than 30 s) paired-tracer dilution technique. In the non-diabetic pancreas L-serine influx was saturable and stimulated by perfusion with exogenous bovine insulin (100 microU/ml). Transport of L-serine and methylaminoisobutyric acid was markedly elevated in pancreata isolated from streptozotocin diabetic rats and insulin partially reversed the stimulation of L-serine transport induced by experimental diabetes. These results suggest that insulin and diabetes modulate the epithelial transport activity for small neutral amino acids in the intact exocrine pancreas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism*
  • Animals
  • Basement Membrane / metabolism
  • Biological Transport, Active
  • Diabetes Mellitus, Experimental / metabolism*
  • Insulin / physiology*
  • Kinetics
  • Male
  • Pancreas / metabolism*
  • Pancreas / ultrastructure
  • Perfusion
  • Rats
  • Rats, Inbred Strains
  • Serine / metabolism*


  • Amino Acids
  • Insulin
  • Serine