The IsTaR 1 serodeme of Trypanosoma brucei: development of a new serodeme

J Parasitol. 1984 Oct;70(5):747-54.


An extensive serodeme of sequentially-isolated antigenic variants of African trypanosomes has been produced from both syringe-passaged and cyclically-transmitted Trypanosoma brucei of the IsTaR 1 clone derived from EATRO 164. The majority of the antigenic variants were isolated from chronically-infected deer mice (Peromyscus leucopus). The pattern of parasitemias during the course of infections initiated with syringe-passaged trypanosomes differed from those initiated with cyclically-transmitted trypanosomes. Trypanosome populations from syringe-passaged (192) and cyclically-transmitted (31) clones were each amplified by growth in lethally-irradiated mice and cryopreserved for retrospective analysis. Five clones derived from a single deer mouse during the first 44 days of infection, and 2 clones derived from an acutely-infected rat were established from these amplified populations. Homogeneous populations were grown in lethally-irradiated rats and mice for antigenic analysis purification of variant-specific glycoprotein. Six of the 7 clones were distinct variants by immunological criteria using antisera derived from whole cells or purified surface glycoproteins. Two clones, one derived from the acutely-infected rat, and the other from the first parasitemia in a chronic infection that was initiated with the former clone, were immunologically identical. Production of these clones established a well-defined serodeme that will allow detailed analysis of antigenic variation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Glycoproteins / analysis*
  • Immune Sera
  • Immunodiffusion
  • Mice
  • Peromyscus
  • Rats
  • Species Specificity
  • Trypanosoma brucei brucei / classification
  • Trypanosoma brucei brucei / immunology*
  • Trypanosoma brucei brucei / pathogenicity
  • Trypanosomiasis, African / parasitology
  • Variant Surface Glycoproteins, Trypanosoma


  • Glycoproteins
  • Immune Sera
  • Variant Surface Glycoproteins, Trypanosoma