Growth of bacteriophage phiX-174 at elevated temperatures

J Gen Virol. 1980 Jul;49(1):41-50. doi: 10.1099/0022-1317-49-1-41.

Abstract

The replication of bacteriophage phiX-174 is impaired at temperatures above 40 degrees C. Mutants (ht) that replicate at high temperature were isolated and partially characterized. Wild-type phiX fails to grow at high temperature because, unlike the mutants, it does not make appreciable amounts of single-stranded (ss)DNA. An unusual form of ssDNA, not found in complete virions, is described.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriophage phi X 174 / genetics
  • Bacteriophage phi X 174 / growth & development*
  • Bacteriophage phi X 174 / metabolism
  • DNA, Single-Stranded / analysis
  • DNA, Single-Stranded / biosynthesis
  • DNA, Viral / analysis
  • DNA, Viral / biosynthesis
  • Hot Temperature
  • Mutation

Substances

  • DNA, Single-Stranded
  • DNA, Viral