Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. II. Characterization of a soluble suppressor of B cell immunoglobulin production

J Immunol. 1981 Mar;126(3):1192-7.


Human peripheral blood mononuclear cells (PBMC) activated with the mitogenic lectin concanavalin A (Con A) elaborate a soluble immune suppressor supernatant (SISS) that contains at least 2 distinct suppressor factors. One of these, SISS-B, inhibits polyclonal B cell immunoglobulin production whereas the other, SISS-T, suppresses T cell proliferation to both mitogens and antigens. The latter mediator is discussed in the companion paper. Characteristics of the human soluble suppressor of B cell immunoglobulin production (SISS-B) include: 1) inhibition by a noncytotoxic mechanism, 2) loss of activity in the presence of the monosaccharide L-rhamnose, 3) appearance within 8 to 16 hr after the addition of Con A, 4) elaboration by cells irradiated with 500 or 2000 rads, 5) production by highly purified T cells, 6) stability at pH 2.5 but instability at 56 degrees C, and 7) m.w. of 60 to 80,000. These data indicate that after Con A activation, selected T cells not only become potent suppressor cells, but also generate a soluble saccharide-specific factor(s) that inhibits polyclonal immunoglobulin production by human B cells.

MeSH terms

  • Acetylglucosamine / pharmacology
  • B-Lymphocytes / immunology*
  • Cell Adhesion
  • Chromatography, Gel
  • Concanavalin A / pharmacology*
  • Humans
  • Immunoglobulins / biosynthesis
  • Lymphocyte Activation*
  • Methylmannosides / pharmacology
  • Pokeweed Mitogens / pharmacology
  • Rhamnose / pharmacology
  • Solubility
  • T-Lymphocytes / immunology
  • T-Lymphocytes, Regulatory / immunology*


  • Immunoglobulins
  • Methylmannosides
  • Pokeweed Mitogens
  • Concanavalin A
  • Rhamnose
  • Acetylglucosamine