Characterization of a soluble suppressor of human B cell immunoglobulin biosynthesis produced by a continuous human suppressor T cell line

J Exp Med. 1981 Jul 1;154(1):156-67. doi: 10.1084/jem.154.1.156.

Abstract

A human suppressor T cell maintained in long-term culture with conditioned medium containing interleukin 2 elaborates a suppressor factor(s) that specifically inhibits human polyclonal B cell immunoglobulin biosynthesis. This soluble immune suppressor supernate of immunoglobulin production (CTC-SISS-B) shares a number of features with the previously described suppressive mediator elaborated by concanavalin A-activated human peripheral T cells (SISS-B) including: (a) the inhibition by a noncytotoxic mechanism, (b) the suppression of immunoglobulin biosynthesis either through direct action on the B cell or indirect action via the monocyte, (c) the loss of inhibition in the presence of the monosaccharide L-rhamnose, (d) the elaboration by cells irradiated with 500 ro 2,000 rad, and (e) molecular weights of 60,000--90,000. Furthermore, the suppression by this mediator appears to be specific for B cell immunoglobulin production in that CTC-SISS B has no effect on T cell proliferation to mitogens, antigens, an allogeneic cells or on T cell-mediated cytotoxicity. These data indicate that one possible mechanism of suppressor T cell inhibition of human immunoglobulin production is via the generation of a lectinlike suppressor lymphokine that interacts with defined saccharide determinants on the cell surface of either the B cell or monocyte.

MeSH terms

  • Animals
  • Antibodies
  • Antibodies, Monoclonal
  • B-Lymphocytes / immunology*
  • Chromatography, Gel
  • Culture Media
  • Cytotoxicity, Immunologic
  • Fucose / pharmacology
  • Galactose / pharmacology
  • Humans
  • Immunoglobulins / biosynthesis*
  • Kinetics
  • Phytohemagglutinins / pharmacology
  • Rabbits
  • Rhamnose / pharmacology
  • Solubility
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Regulatory / immunology*
  • Thymidine / metabolism
  • Time Factors

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Culture Media
  • Immunoglobulins
  • Phytohemagglutinins
  • Fucose
  • Rhamnose
  • Thymidine
  • Galactose