Effects of floury-2 locus on zein accumulation and RNA metabolism during maize endosperm development

Biochem Genet. 1978 Feb;16(1-2):27-38. doi: 10.1007/BF00484382.


Zein accumulation patterns during mutant and normal maize endosperm development were determined. Accompanying an increase in the number of floury-2 alleles present in the endosperm was a well-defined stepwise depression in zein accumulation. Analysis of the zein accumulated in endosperms containing zero, one, two, and three doses of the floury-2 allele by sodium dodecylsulfate--polyacrylamide gel electrophoresis revealed a proportionate reduction in the two major zein components, Z1 and Z2. In contrast, the relative proportions of the minor zein bands were altered. Membrane-bound polysomes isolated from kernels of floury-2 and normal maize were predominantly large size classes. The presence of increasing numbers of the floury-2 allele in the endosperm decreased recovery of membrane-bound polysomal material in a stepwise fashion. However, major alterations in polysome size-class distributions were not observed. The reduction in membrane-bound polysome material correlated linearly with reductions in in vitro zein synthesis and in vivo zein accumulation.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Genes*
  • Mutation
  • Protein Precursors / genetics
  • Protein Precursors / metabolism
  • RNA / genetics
  • RNA / metabolism*
  • Zea mays / genetics*
  • Zea mays / metabolism
  • Zein / biosynthesis
  • Zein / genetics*


  • Protein Precursors
  • RNA
  • Zein