Glutathione depletion in tissues after administration of buthionine sulphoximine

Int J Radiat Oncol Biol Phys. 1984 Aug;10(8):1261-4. doi: 10.1016/0360-3016(84)90329-8.

Abstract

Buthionine sulphoximine (BSO) an inhibitor of glutathione (GSH) biosynthesis, was administered to mice in single and repeated doses of 0.5, 1 and 5 mmol kg-1 (i.p.). The resultant pattern of GSH depletion was studied in liver, kidney, skeletal muscle and three types of murine tumor. Liver and kidney exhibited a rapid depletion to GSH levels of ca. 20% of controls after single doses of 1-5 mmol kg-1 BSO. Muscle was depleted to a similar level, but at a slower rate after a single dose. All three tumors required repeated administration of BSO over several days to obtain a similar degree of depletion to that shown in the other tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Buthionine Sulfoximine
  • Female
  • Glutathione / metabolism*
  • Kidney / metabolism
  • Liver / metabolism
  • Methionine Sulfoximine / analogs & derivatives*
  • Methionine Sulfoximine / pharmacology
  • Mice
  • Muscles / metabolism
  • Neoplasm Transplantation
  • Neoplasms, Experimental / metabolism
  • Time Factors

Substances

  • Methionine Sulfoximine
  • Buthionine Sulfoximine
  • Glutathione