Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay

Biochim Biophys Acta. 1978 Apr 26;533(2):525-9. doi: 10.1016/0005-2795(78)90398-7.


A quantitative assay method for protein is described which is based on the color change occurring in Coomassie Brilliant Blue G-250 when it binds to protein. This modification of two similar procedures further increases the sensitivity, simplicity, and stability of the protein-dye binding assay over those of other commonly used assays for protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Colorimetry / methods
  • Indicators and Reagents
  • Microchemistry
  • Proteins / analysis*


  • Indicators and Reagents
  • Proteins