A simple in vitro technique to measure the release of endogenous dopamine and dihydroxyphenylacetic acid from striatal tissue using high performance liquid chromatography with electrochemical detection

J Neurosci Methods. 1984 May;11(1):19-28. doi: 10.1016/0165-0270(84)90004-9.

Abstract

An easily constructed, inexpensive and simple system is described for the superfusion of neural tissue. To characterize the system, the dynamics of endogenous dopamine and dihydroxyphenylacetic acid release from striatal tissue were studied before and after exposure to potassium, amphetamine or cocaine.

MeSH terms

  • 3,4-Dihydroxyphenylacetic Acid / metabolism*
  • Amphetamine / pharmacology
  • Animals
  • Chromatography, High Pressure Liquid / methods*
  • Cocaine / pharmacology
  • Corpus Striatum / drug effects
  • Corpus Striatum / metabolism*
  • Dopamine / metabolism*
  • Electrochemistry
  • Male
  • Phenylacetates / metabolism*
  • Potassium / pharmacology
  • Rats
  • Temperature

Substances

  • Phenylacetates
  • 3,4-Dihydroxyphenylacetic Acid
  • Amphetamine
  • Cocaine
  • Potassium
  • Dopamine