Poly(ADP-ribose) metabolism appears normal in EM9, a mutagen-sensitive mutant of CHO cells

Mutat Res. 1984 Sep;128(2):213-20. doi: 10.1016/0027-5107(84)90109-x.

Abstract

EM9 is a mutagen-sensitive CHO cell whose phenotype resembles that of normal CHO cells exposed to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) synthesis. This phenotype suggested that EM9 might be defective in poly(ADP-ribose) metabolism, but we now cannot find any abnormality in the synthesis or in the degradation of poly(ADP-ribose) in permeabilized EM9 cells. Thus the effects of 3-aminobenzamide on wild-type cells may be due to the inhibition of processes other than poly(ADP-ribose) synthesis. 3-Aminobenzamide enhances the cytotoxicity of EMS toward EM9 and control cells to the same degree.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Cell Membrane / metabolism
  • Cricetinae
  • Cricetulus
  • Deoxyribonucleases / metabolism
  • Deoxyuridine / analogs & derivatives
  • Deoxyuridine / toxicity
  • Detergents / pharmacology
  • Ethyl Methanesulfonate / toxicity
  • Female
  • Isomerism
  • Kinetics
  • Lysophosphatidylcholines / pharmacology
  • Mutagenicity Tests
  • Mutagens / toxicity*
  • Mutation*
  • NAD / metabolism
  • Nucleoside Diphosphate Sugars / metabolism*
  • Octoxynol
  • Ovary
  • Poly Adenosine Diphosphate Ribose / metabolism*
  • Polyethylene Glycols / pharmacology

Substances

  • Detergents
  • Lysophosphatidylcholines
  • Mutagens
  • Nucleoside Diphosphate Sugars
  • NAD
  • Poly Adenosine Diphosphate Ribose
  • Polyethylene Glycols
  • 2'-chloro-2'-deoxyuridine
  • Octoxynol
  • Ethyl Methanesulfonate
  • Deoxyribonucleases
  • Deoxyuridine