Acidification of interstitial fluid in hippocampal formation caused by seizures and by spreading depression

Brain Res. 1984 Oct 8;311(1):186-8. doi: 10.1016/0006-8993(84)91416-1.


Changes in the pH of interstitial fluid were measured with H+-selective double-barreled micropipette electrodes in fascia dentata of urethane-anesthetized rats. Paroxysmal afterdischarges provoked by repetitive stimulation of an afferent fiber tract brought in their wake acidification by 0.07 to 0.2 pH units. Spreading depression caused acidification by 0.2-0.5 pH units. Acid shifts were often preceded by transient alkalinization. Acidification is attributed to the production of CO2 and of other acid metabolites.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cortical Spreading Depression*
  • Electric Stimulation
  • Evoked Potentials, Somatosensory
  • Extracellular Space / physiology*
  • Hippocampus / physiology*
  • Hippocampus / physiopathology
  • Hydrogen-Ion Concentration
  • Male
  • Rats
  • Seizures / physiopathology*