Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization

Anal Biochem. 1984 Aug 15;141(1):101-9. doi: 10.1016/0003-2697(84)90431-7.


The use of N-methyl-N-(t-butyldimethylsilyl)trifluoroacetamide to prepare the t-butyldimethylsilyl derivatives of a number of organic compounds (selected amino acids, alpha-keto acids, ketone bodies, free fatty acids, urea, glycerol, lactate, and pyruvate) is reported. These derivatives are particularly useful for gas chromatographic/mass spectrometric analysis involving the use of stable isotopes and selected ion monitoring, since a peak of sufficient abundance at 57 mass/charge units below the molecular ion was always present, and was the result of the loss of one t-butyl group. In each case, this fragment contained the entire skeleton of the original compound, which permitted easy analysis using electron-impact ionization of these compounds alone or when labeled with stable isotopes in any nonexchangeable position.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / blood
  • Blood Chemical Analysis / methods*
  • Fatty Acids, Nonesterified / blood
  • Gas Chromatography-Mass Spectrometry
  • Glycerol / blood
  • Keto Acids / blood
  • Ketone Bodies / blood
  • Lactates / blood
  • Lactic Acid
  • Organosilicon Compounds*
  • Pyruvates / blood
  • Silicon*
  • Urea / blood


  • Amino Acids
  • Fatty Acids, Nonesterified
  • Keto Acids
  • Ketone Bodies
  • Lactates
  • Organosilicon Compounds
  • Pyruvates
  • t-butyldimethylsilyl compounds
  • Lactic Acid
  • Urea
  • Glycerol
  • Silicon