Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

Anal Biochem. 1984 Aug 15;141(1):110-5. doi: 10.1016/0003-2697(84)90432-9.


JH imple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-13C2]acetoacetate is described. Mole percent enrichment of beta-[13C2]hydroxybutyrate and [13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Hydroxybutyric Acid
  • Acetoacetates / blood
  • Animals
  • Carbon Isotopes
  • Dogs
  • Gas Chromatography-Mass Spectrometry
  • Hydroxybutyrates / blood
  • Ketone Bodies / blood
  • Ketone Bodies / metabolism*


  • Acetoacetates
  • Carbon Isotopes
  • Hydroxybutyrates
  • Ketone Bodies
  • acetoacetic acid
  • 3-Hydroxybutyric Acid