Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from pseudomonas spec. CBS 3

Biochem Biophys Res Commun. 1984 Oct 15;124(1):178-82. doi: 10.1016/0006-291x(84)90933-1.

Abstract

4-Chlorobenzoate is dehalogenated by 4-chlorobenzoate dehalogenase from Pseudomonas spec. CBS 3 to form 4-hydroxybenzoate. In 18O enriched water the hydroxygroup of 4-hydroxybenzoate is quantitatively labelled with 18O. This result clearly shows, that 4-chlorobenzoate dehalogenase catalyzes the hydrolytic cleavage of the halogen-carbon bond, without the involvement of molecular oxygen, a reaction not yet described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Gas
  • Hydrolases / metabolism*
  • Hydroxybenzoates / metabolism*
  • Isotope Labeling / methods
  • Kinetics
  • Mass Spectrometry
  • Oxygen Isotopes
  • Parabens*
  • Pseudomonas / metabolism*
  • Water / metabolism

Substances

  • Hydroxybenzoates
  • Oxygen Isotopes
  • Parabens
  • Water
  • Hydrolases
  • 4-chlorobenzoate dehalogenase
  • 4-hydroxybenzoic acid