Sulfation in isolated enterocytes of guinea pig: dependence on inorganic sulfate

Biochem Pharmacol. 1984 Nov 1;33(21):3353-6. doi: 10.1016/0006-2952(84)90105-9.


Isolated intestinal epithelial cells of the guinea-pig were used to study uptake and metabolism of inorganic sulfate in the jejunum (proximal cells) and ileum (distal cells). Proximal enterocytes accumulated sulfate 1.5-fold and distal enterocytes 3.1-fold. Accumulation was almost linearly related to substrate concentrations up to 5 mM. In proximal cells, despite their lower intracellular sulfate levels, sulfate incorporation into acid-precipitable material and sulfate conjugation of 1-naphthol were faster than in distal cells. Formation of 1-naphthyl sulfate increased with extracellular sulfate concentrations up to 1 and 3 mM sulfate in distal and proximal enterocytes, respectively. The data suggest that the extent of intestinal sulfation of phenolic compounds may be enhanced by oral administration of sulfate.

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Guinea Pigs
  • In Vitro Techniques
  • Intestinal Mucosa / metabolism*
  • Intestines / cytology
  • Male
  • Naphthols / metabolism
  • Sulfates / metabolism*
  • Time Factors


  • Naphthols
  • Sulfates
  • 1-naphthol