The estrogenic activity of DDT: the in vitro induction of an estrogen-inducible protein by o,p'-DDT

Toxicol Appl Pharmacol. 1984 Dec;76(3):537-43. doi: 10.1016/0041-008x(84)90358-2.


Induced protein is a commonly measured marker for estrogenic action. The induction of induced protein by o,p'-DDT was studied in an in vitro system. Nuclear levels of estrogen receptor translocated by o,p'-DDT correlated highly with induced protein induction, and the time course for induced protein induction was consistent with an estrogen receptor-mediated mechanism. While the maximum amount of induced protein produced by o,p'-DDT was less than after 17 beta-estradiol exposure, the induced protein formed by each compound was indistinguishable on nondenaturing and denaturing polyacrylamide gels. Also, it was shown that o,p'-DDT does not cause additional induction of induced protein over that seen with maximum levels of 17 beta-estradiol, further supporting the premise that these compounds share a common pathway in stimulating the synthesis of induced protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DDT / pharmacology*
  • Electrophoresis, Polyacrylamide Gel
  • Estradiol / pharmacology
  • Estrogens, Non-Steroidal*
  • Female
  • In Vitro Techniques
  • Protein Biosynthesis*
  • Rats
  • Receptors, Estrogen / drug effects
  • Uterus / drug effects*
  • Uterus / metabolism


  • Estrogens, Non-Steroidal
  • Receptors, Estrogen
  • Estradiol
  • DDT
  • o,p'-DDT