A Second Site-Specific Restriction Endonuclease From Staphylococcus Aureus

Nucleic Acids Res. 1978 Apr;5(4):1153-63. doi: 10.1093/nar/5.4.1153.

Abstract

A site-specific restriction endonuclease has been isolated from Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequence 5'--G-G-N-C-C--3' and cleaves as indicated by the arrows. The enzyme 3'--C-C-N-G-G--5' cleaves adenovirus type 5 and lambda DNA many times, SV40 DNA 10 times and 0X174 RF DNA 2 times. Evidence is presented that the enzyme is involved in biological restriction-modification.

MeSH terms

  • Base Sequence
  • Binding Sites
  • DNA, Viral / metabolism
  • Endonucleases / isolation & purification
  • Endonucleases / metabolism*
  • Staphylococcus aureus / enzymology*
  • Substrate Specificity

Substances

  • DNA, Viral
  • Endonucleases