Immunological activities of rat lymphocytes. I. Mitogenic responses and surface markers of lymphocytes from normal, thymectomized and B rats

Int Arch Allergy Appl Immunol. 1976;52(1-4):32-41.

Abstract

Lymphocytes from the peripheral blood, thoracic duct, spleen and lymph nodes of normal, thymectomized (TX) and thymectomized lethally irradiated marrow reconstituted (TXBM or B rats) Lewis rats were studied for their ability to proliferate in vitro in the presence of Con A or PHA. At the same time the lymphoid tissues of these animals were examined for the presence of B cells or T cells by immunofluorescence staining with antiserums to rat immunoglobulins or rat brain antigens (ARBS), respectively. The specificity of ARBS fort T cells was first established in both cytotoxicity and immunofluoresecnce studies with thymocytes as well as pruified T and B cells. In various lymphoid tissues from TX and B rats, cells carrying brain antigens were found which were unable to respond to Con A and PHA. Thymectomy has a lesser effect on the response to Con A that to PHA; this was particularly true in the thoracic duct where, following thymectomy, lymphocytes continued to respond to Con A but lost their ability to respond to PHA. This suggests that the response to PHA and Con A may belong to different cells' subsets but that the ability to respond to either one of these mitogens may be impaired in cells carrying known T cell markers.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • Bone Marrow Cells
  • Bone Marrow Transplantation
  • Brain / immunology*
  • Concanavalin A / pharmacology
  • Epitopes*
  • Fluorescent Antibody Technique
  • Lectins / pharmacology
  • Lymph Nodes / immunology
  • Lymphocyte Activation*
  • Rats
  • Spleen / immunology
  • T-Lymphocytes / immunology*
  • Thymectomy
  • Thymus Gland / immunology*

Substances

  • Epitopes
  • Lectins
  • Concanavalin A