The contribution of on-bipolar cells to the electroretinogram of rabbits and monkeys. A study using 2-amino-4-phosphonobutyrate (APB)

Vision Res. 1984;24(12):1841-6. doi: 10.1016/0042-6989(84)90016-6.


We have obtained electroretinograms (ERGs) from rabbits and macaque monkeys after vitreal administration of 2-amino-4-phosphonobutyrate (APB), which selectively blocks light-responsiveness in retinal on-bipolar cells. Microelectrode recordings from central visual structures provided an independent measure of the drug effects. In rabbits, APB blocked the b-wave, a sustained corneal-positive potential, and a transient corneal-negative off-response. In monkeys, APB abolished the b-wave but had little or no effect on the d-wave. The d-wave could be eliminated, however, by the use of broadband blue stimuli. The results are discussed in light of recent hypotheses about the cellular origins of the ERG.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminobutyrates / pharmacology*
  • Animals
  • Dose-Response Relationship, Drug
  • Electroretinography
  • Macaca mulatta
  • Neurons / drug effects
  • Neurons / physiology
  • Rabbits
  • Retina / cytology*
  • Retina / drug effects


  • Aminobutyrates
  • 2-amino-4-phosphonobutyric acid