Three-dimensional reconstruction of rigor insect flight muscle from tilted thin sections

Nature. 1984;310(5975):285-91. doi: 10.1038/310285a0.


Rigor cross-bridges show two conformations paired within each 38.7-nm axial repeat. The two forms may express two stages of the cross-bridge cycle during contraction. Differing numbers of myosin heads per cross-bridge and associated helical changes in the thin filament distinguish the two forms and suggest that both myosin heads usually bind to a single thin filament.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism
  • Animals
  • Coleoptera / ultrastructure*
  • Cytoskeleton / metabolism
  • Cytoskeleton / ultrastructure
  • Flight, Animal
  • Macromolecular Substances
  • Microscopy, Electron
  • Models, Biological
  • Muscle Contraction*
  • Muscles / ultrastructure*
  • Myosins / metabolism
  • Protein Conformation
  • Troponin / metabolism
  • X-Ray Diffraction


  • Actins
  • Macromolecular Substances
  • Troponin
  • Myosins