Glucagon-related peptides in the human gastrointestinal mucosa

Diabetologia. 1984 Mar;26(3):223-8. doi: 10.1007/BF00252412.

Abstract

We studied the chromatographic profile and the distribution of glucagon-related peptides in the human gastrointestinal mucosa, using radioimmunoassays directed against the glucagon 6-15 and 19-29 sequences, and against the glicentin sequences 15-30 and 61-69, and a radioreceptor assay for glucagon. Very small amounts of glucagon-related peptides were found in the gastric mucosa, whereas at least four different components could be identified in the distal intestine. One component (mean concentration 130 pmol/g ileal mucosa) is similar to porcine glicentin for size and C-terminal extension, but differs from the glucagon part of the molecule in the N-terminal extension. A second component (mean concentration 131 pmol/g) is probably identical to porcine peak II enteroglucagon (glicentin 33-69), and a third component (7.9 pmol/g) seems to be identical with glucagon. A fourth component containing the glucagon sequence plus an N-terminal extension was also identified (1.7 pmol/g). Thus the human intestinal mucosa contains large amounts of peptides containing the glucagon sequence; at least one of these probably also possesses glucagon-like bioactivity. The proposed structures of the four components are consistent with the base sequence of the first half of the human glucagon gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gastric Mucosa / analysis*
  • Gastrointestinal Hormones / analysis*
  • Glucagon / analysis*
  • Glucagon / immunology
  • Glucagon-Like Peptides / analysis*
  • Humans
  • Intestinal Mucosa / analysis*
  • Peptides / analysis*
  • Proglucagon
  • Protein Precursors / analysis
  • Radioimmunoassay
  • Radioligand Assay

Substances

  • Gastrointestinal Hormones
  • Peptides
  • Protein Precursors
  • Proglucagon
  • Glucagon-Like Peptides
  • glucagon-like-immunoreactivity
  • Glucagon