Uridine as an active component of sleep-promoting substance: its effects on nocturnal sleep in rats

Neurosci Res. 1984 Aug;1(4):243-52. doi: 10.1016/s0168-0102(84)80003-6.


A 10-h intraventricular infusion of 10 pmol of uridine from 19.00 to 05.00 h resulted in significant increases in sleep in otherwise saline-infused male rats (n = 8) during the environmental dark period (20.00-08.00 h). Increments of slow wave sleep (SWS) and paradoxical sleep (PS) were 21.0% and 68.1%, respectively, of the baseline value. This was due to increases in the frequencies of both SWS and PS episodes but not to their durations. Similar increases occurred the first recovery night under saline infusion, but sleep amounts returned to the baseline levels the second night. Brain temperature was not affected by uridine administration. A small dose of uridine (1 pmol/10 h) exerted no effect (n = 6) while larger doses (100 and 1000 pmol/10 h, each n = 5) resulted in slight but insignificant increases in SWS and PS. The 1000-pmol uridine administration seemed to be non-physiological since it brought about irregularities in locomotor activity and sleep-waking rhythms. Thus, authentic uridine exhibited the same sleep-enhancing effects as a naturally occurring active component of sleep-promoting substance, which was recently identified with uridine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemical Phenomena
  • Chemistry
  • Circadian Rhythm / drug effects
  • Delta Sleep-Inducing Peptide
  • Hypnotics and Sedatives / analysis*
  • Injections, Intraventricular
  • Male
  • Oligopeptides / analysis*
  • Rats
  • Rats, Inbred Strains
  • Sleep / drug effects*
  • Stimulation, Chemical
  • Uridine / analysis*
  • Uridine / pharmacology


  • Hypnotics and Sedatives
  • Oligopeptides
  • Delta Sleep-Inducing Peptide
  • Uridine