Abstract
The inhibitory effects of 6-amidino-2-naphthyl 4-guanidinobenzoate (FUT-175) on the activities of factor B, factor D and cobra venom factor (CVF) X Bb were examined. FUT-175 bound specifically to the Bb fragment of factor B or CVF X Bb. FUT-175 was a non-competitive inhibitor of the esterolysis of L-leucyl-L-alanyl-L-arginine naphthylester by factor B and CVF X Bb. FUT-175 also inhibited the haemolytic activity of factor B, the C3 convertase activity of CVF X Bb and the factor B-cleaving activity of factor D. The concentration of FUT-175 causing 50% inhibition of these activities was 10(-5) - 10(-4)M.
MeSH terms
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Animals
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Benzamidines
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Complement Activation / drug effects*
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Complement Factor B / antagonists & inhibitors
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Complement Factor D / antagonists & inhibitors
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Complement Inactivator Proteins / pharmacology*
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Complement Pathway, Alternative / drug effects*
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Dose-Response Relationship, Drug
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Elapid Venoms / antagonists & inhibitors
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Electrophoresis, Polyacrylamide Gel
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Guanidines / pharmacology*
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Hemolysis / drug effects
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Humans
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In Vitro Techniques
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Rabbits
Substances
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Benzamidines
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Complement Inactivator Proteins
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Elapid Venoms
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Guanidines
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cobra venom factor
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CFD protein, human
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Complement Factor D
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Complement Factor B
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nafamostat