Determination of immunoreactive trypsin, pancreatic elastase and chymotrypsin in extracts of human feces and ileostomy drainage

Digestion. 1983;27(1):8-15. doi: 10.1159/000198913.


The total daily amount of extractable cationic trypsin, chymotrypsin, and pancreatic elastase 2 in feces and ileostomy fluids has been studied in normal individuals and healthy colectomized subjects. Quantitation was performed using immunological assays with polyethylene glycol as a fecal marker. The extractable amount of each of these enzymes in the feces of normal individuals was less than 1 mg/24 h. However, in fecal extracts from antibiotic-treated normal individuals a 100-fold increase in immunoreactive cationic trypsin was observed, while chymotrypsin and elastase 2 were only 2- to 3-fold higher. In extracts from ileostomy fluids cationic trypsin, elastase, and chymotrypsin all showed mean values in the order of 50-200 mg/24 h. The characterization of the immunoreactivity of pancreatic proteases showed no qualitative differences when measured in duodenal juice or fecal and ileostomy extracts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anti-Bacterial Agents / pharmacology
  • Body Fluids / enzymology
  • Chromatography, Gel
  • Chymotrypsin / analysis*
  • Colon / drug effects
  • Cryoprotective Agents
  • Feces / enzymology*
  • Humans
  • Ileostomy*
  • Middle Aged
  • Pancreatic Elastase / analysis*
  • Polyethylene Glycols
  • Radioimmunoassay
  • Trypsin / analysis*


  • Anti-Bacterial Agents
  • Cryoprotective Agents
  • Polyethylene Glycols
  • Chymotrypsin
  • Pancreatic Elastase
  • Trypsin