Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 80 (10), 2866-9

Molecular Cloning of Human Gastrin cDNA: Evidence for Evolution of Gastrin by Gene Duplication

Molecular Cloning of Human Gastrin cDNA: Evidence for Evolution of Gastrin by Gene Duplication

E Boel et al. Proc Natl Acad Sci U S A.

Abstract

An oligo(dT)-primed cDNA copy of the mRNA coding for the human gastrin precursor was constructed from poly(A)-containing RNA from a human pancreatic, gastrin-producing tumor (a gastrinoma). The cDNA was inserted into the Pst I endonuclease site of plasmid pBR322 by the use of the poly(dC) and poly(dG) tailing procedure. Clones containing gastrin sequences were selected by hybridization to a purified single-stranded 32P-labeled gastrin cDNA probe. This probe was constructed with gastrinoma mRNA as template. As primer for the cDNA synthesis, we used a synthetic oligonucleotide mixture, d(AG-A-A-AG-T-C-C-A-T-C-C-A), corresponding to the gastrin-specific amino acid sequence Trp-Met-Asp-Phe. In this way we determined the nucleotide sequence of the entire coding region (303 nucleotides), the entire 3' untranslated region (102 nucleotides), and 8 nucleotides of the 5' untranslated region. A striking homology between parts of the coding region suggests that evolution of the gastrin gene has involved a gene duplication.

Similar articles

See all similar articles

Cited by 39 articles

See all "Cited by" articles

References

    1. J Mol Biol. 1966 Mar;16(1):118-33 - PubMed
    1. Nature. 1966 Feb 5;209(5023):583-5 - PubMed
    1. Gastroenterology. 1970 May;58(5):609-15 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. Biochim Biophys Acta. 1972 Dec 28;285(2):364-72 - PubMed

Publication types

Associated data

Feedback