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, 66 (4), 299-308

Large Scale Isolation of Nuclei and Nucleoli From Vitellogenic Oocytes of Xenopus Laevis

Large Scale Isolation of Nuclei and Nucleoli From Vitellogenic Oocytes of Xenopus Laevis

F Scalenghe et al. Chromosoma.

Abstract

A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous RNA polymerase activity as well as a high level of activity with an exogenously added template.

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References

    1. Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1077-82 - PubMed
    1. J Morphol. 1972 Feb;136(2):153-79 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1502-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Apr;57(4):885-92 - PubMed
    1. Cell. 1977 Sep;12(1):175-82 - PubMed
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