Sequence of a complete chicken delta-crystallin cDNA

Proc Natl Acad Sci U S A. 1984 May;81(9):2611-5. doi: 10.1073/pnas.81.9.2611.


A full-length chicken delta-crystallin cDNA (p delta Cr17) was cloned and subjected to sequence analysis. The cDNA was shown to be full-length by both primer extension and S1 and mung bean nuclease experiments. Thus, the complete amino acid sequence of delta-crystallin is now available. The delta-crystallin polypeptide has a molecular mass of 48,542 daltons, as expected from its behavior on NaDodSO4/polyacrylamide gels. delta-Crystallin has one tryptophan, no cysteines, a prevalence of leucines (15%), and a paucity of aromatic residues. High alpha-helical content throughout the protein was predicted from the amino acid sequence. Nucleic acid sequence analysis suggests that delta-crystallin gene 1 encodes the mRNA that gave rise to the cDNA clone. These data provide a basis for the detailed analysis of the two delta-crystallin genes.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens
  • Cloning, Molecular
  • Crystallins / genetics*
  • DNA / genetics
  • Lens, Crystalline / physiology*
  • Protein Conformation
  • RNA, Messenger / genetics


  • Crystallins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/J00843