Selective killing of leukemic cells by merocyanine 540-mediated photosensitization

Proc Natl Acad Sci U S A. 1984 Dec;81(23):7584-7. doi: 10.1073/pnas.81.23.7584.

Abstract

In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of lethally irradiated syngeneic mice. This simple and rapid procedure may find an application in the purging of tumor cells from autologous bone marrow grafts.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow / drug effects
  • Bone Marrow / pathology*
  • Bone Marrow / radiation effects
  • Cell Survival / drug effects
  • Colony-Forming Units Assay
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / radiation effects
  • Leukemia L1210 / pathology*
  • Light
  • Mice
  • Pyrimidinones / toxicity*
  • Radiation-Sensitizing Agents / toxicity*

Substances

  • Pyrimidinones
  • Radiation-Sensitizing Agents
  • merocyanine dye