In vivo/in vitro labeling of red blood cells with 99mTc

Eur J Nucl Med. 1983;8(5):218-22. doi: 10.1007/BF00253769.


Red blood cells (RBC) were labeled with 99mTc in vitro after in vivo pretreatment with a stannous solution. The whole in vivo/in vitro procedure took 30-40 min. Pretinning was carried out by injection of either 6 mg DTPA and 0.6 mg Sn++ (Sn-DTPA) or 5 mg pyrophosphate and 0.7 mg Sn++ (Sn-PYP). The results of 1,356 patients were evaluated. The labeling yield was 89.7% (mean) after pretinning with Sn-DTPA and 88.2% following Sn-PYP pretreatment, the median values being 94% and 92%, respectively. The new method was successfully used in over 2,000 patients who had a radionuclide-ventriculography and in 38 patients studied for localization of occult gastrointestinal bleeding. Adverse side effects have never been observed.

Publication types

  • Comparative Study

MeSH terms

  • Erythrocytes* / metabolism
  • Gastrointestinal Hemorrhage / blood
  • Gastrointestinal Hemorrhage / diagnostic imaging*
  • Heart Function Tests / methods*
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Radionuclide Imaging
  • Technetium*
  • Tin / blood


  • Technetium
  • Tin