Chromosome abnormality in a human TCGF-dependent T-lymphocyte culture generated against autologous melanoma cells in vitro

Cancer Genet Cytogenet. 1983 Nov;10(3):277-82. doi: 10.1016/0165-4608(83)90056-0.

Abstract

Six T-cell growth promoting factor (TCGF)-dependent T-cell cultures and phytohemagglutinin-stimulated peripheral blood lymphocytes from a patient with metatastic malignant melanoma were karyotyped. Three of the TCGF-dependent cultures were noncloned, started after in vitro sensitization against autologous malignant melanoma cells in tissue culture. A fourth was a subculture of one of these. The two remaining T-cell cultures were generated by in vitro sensitization of the same individual's lymphocytes against allogeneic Epstein-Barr virus-transformed B-cell lines. One of the cultures (AV7) and its subculture (AV/LDP39) showed a 17q+ anomaly in 100% of metaphases studied. All other TCGF-dependent T-cell cultures and fresh blood lymphocytes had normal karyotypes. The possible mode of origin of the abnormality and its significance are discussed.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, Surface / analysis
  • Cells, Cultured
  • Chromosome Aberrations*
  • Chromosome Disorders*
  • Cytotoxicity, Immunologic
  • Humans
  • Immunotherapy
  • Interleukin-2 / pharmacology*
  • Karyotyping
  • Lymphocyte Activation
  • Male
  • Melanoma / immunology*
  • Melanoma / therapy
  • Phenotype
  • Rosette Formation
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*

Substances

  • Antigens, Surface
  • Interleukin-2