Posttranslational modification of human T-cell growth factor

Biochem Biophys Res Commun. 1983 Nov 15;116(3):1049-55. doi: 10.1016/s0006-291x(83)80248-4.

Abstract

Amino-terminal sequence analysis of human T-cell growth factor indicated that the amino acid in position 3 of the polypeptide chain was modified. Examination of the N-terminal octapeptide using the amino acid analyzer and mass spectrometry demonstrated that position 3 was a threonine which was linked to N-acetyl-D-galactosamine. This site of glycosylation is of practical significance since it appears to play a role in the selectivity of a monoclonal antibody for the factor.

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Glycoproteins / genetics
  • Hexosamines / analysis
  • Humans
  • Interleukin-2 / genetics*
  • Mass Spectrometry
  • Peptide Fragments / analysis
  • Protein Processing, Post-Translational*
  • T-Lymphocytes
  • Trypsin

Substances

  • Glycoproteins
  • Hexosamines
  • Interleukin-2
  • Peptide Fragments
  • Trypsin