Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines

Cell. 1984 Feb;36(2):319-27. doi: 10.1016/0092-8674(84)90225-3.

Abstract

We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional gel electrophoresis. Furthermore, the I-Ak molecules on L cells can act as targets for the allogenic T-cell killing of the transformed L cells. The I-Ak molecules in both mouse fibroblasts and hamster B cells can present certain antigens to T-cell helper hybridomas. Thus only class II molecules are required to convert the nonantigen-presenting cell. Accordingly, it will be possible to dissect the structure-function relationships existing between Ia molecules, foreign antigen, and T-cell receptor molecules by in vitro site-directed mutagenesis and gene transfer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • B-Lymphocytes / immunology*
  • Cell Line
  • Cricetinae
  • Cytotoxicity, Immunologic
  • Electrophoresis, Polyacrylamide Gel
  • Genes*
  • H-2 Antigens / genetics*
  • H-2 Antigens / isolation & purification
  • Hybridomas / immunology
  • L Cells / immunology
  • Lymphocyte Activation
  • Mice
  • T-Lymphocytes / immunology

Substances

  • Antibodies, Monoclonal
  • H-2 Antigens