Matrix Gla protein, a new gamma-carboxyglutamic acid-containing protein which is associated with the organic matrix of bone

Biochem Biophys Res Commun. 1983 Dec 28;117(3):765-71. doi: 10.1016/0006-291x(83)91663-7.


A new protein has been isolated from CaCl2/urea extracts of demineralized bovine bone matrix. This protein has five to six residues of the vitamin K-dependent amino acid, gamma-carboxyglutamic acid (Gla), and we have accordingly designated it matrix Gla protein. Matrix Gla protein is a 15,000 dalton protein whose amino acid composition includes a single disulfide bond. The absence of 4-hydroxyproline in matrix Gla protein demonstrates that it is not a precursor to bone Gla protein, 5,800 dalton protein which has a residue of 4-hydroxyproline at position 9 in its sequence. Matrix Gla protein also does not cross-react with antibodies raised against bone Gla protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Carboxyglutamic Acid / isolation & purification*
  • Amino Acids / isolation & purification
  • Animals
  • Bone and Bones / analysis*
  • Calcium-Binding Proteins / isolation & purification*
  • Cattle
  • Chemical Phenomena
  • Chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Glutamates / isolation & purification*
  • Osteocalcin
  • Radioimmunoassay
  • Sodium Dodecyl Sulfate


  • Amino Acids
  • Calcium-Binding Proteins
  • Glutamates
  • Osteocalcin
  • Sodium Dodecyl Sulfate
  • 1-Carboxyglutamic Acid