HLA-DC antigens can serve as recognition elements for human cytotoxic T lymphocytes

Eur J Immunol. 1984 Apr;14(4):299-304. doi: 10.1002/eji.1830140404.

Abstract

The specificity of four cytotoxic T lymphocyte (CTL) clones which recognize class II major histocompatibility complex (MHC) antigens was analyzed. All clones recognized antigens associated with the serologically defined HLA-DRw6 specificity. The activity of two of these clones, JR-2-2 and JR-2-10, could be inhibited by a monoclonal antibody Q 5/13 specific for a monomorphic determinant present on HLA-DR. In contrast, the activity of the two other CTL clones, JR-2-19 and JR-2-26, was not blocked by Q 5/13, but by a new monoclonal reagent, SPV-L3. This latter monoclonal antibody precipitated a two-chain structure of 28 kDa and 33 kDa and reacts with a monomorphic determinant. The molecular weight of the polypeptides precipitated with SPV-L3 was slightly less than those precipitated with a HLA-DR-specific monoclonal reagent. In addition two-dimensional gel electrophoresis showed that the antigen precipitated by SPV-L3 differed in charge from those precipitated with the anti-HLA-DR antibody. These results indicate that SPV-L3 recognizes a class II MHC product different from HLA-DR. This observation was confirmed by partial amino acid sequence analysis of the two chains which revealed that the molecule precipitated by SPV-L3 is homologous to HLA-DC/DS molecules. Therefore this report provides the first evidence that human cytotoxic T cells can recognize HLA-DC/DS antigens.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Clone Cells
  • Electrophoresis, Polyacrylamide Gel
  • HLA-DQ Antigens
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Immune Sera
  • Lymphocyte Activation
  • Phytohemagglutinins / immunology
  • Rabbits / immunology
  • Sodium Dodecyl Sulfate
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antibodies, Monoclonal
  • HLA-DQ Antigens
  • Histocompatibility Antigens Class II
  • Immune Sera
  • Phytohemagglutinins
  • Sodium Dodecyl Sulfate