New approach to evaluation of proteinuric states

Clin Chem. 1977;23(4):666-70.


We evaluated the use of immunonephelometric methods for measuring specific urinary proteins. Using a nephelometer to detect light scattering (angle, 31 degrees), we measured some proteins immunonephelometrically in serum and aliquots of 24-h urines from 50 apparently healthy children, ages 2-17 years. The mean urinary excretion rate (mg/24h) and the range of values was: for albumin 5.5 (range, 0-13.3), for transferrin 0.5 (0-1.9, for IgG 3.3 0-12), and for alpha 2-macroglobulin 0.6 (0-2.3). Direct comparison of the values for pathological urines with those for a reference population may offer more meaningful information concerning the integrity of the glomerular basement membrane than is provided by protein selectivity indices, and measuring a plasma protein such as albumin in urine may better define pathological proteinuria.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Albuminuria / diagnosis
  • Child
  • Child, Preschool
  • Evaluation Studies as Topic
  • Humans
  • Immunologic Techniques
  • Nephrotic Syndrome / urine
  • Proteinuria / surgery*
  • Scattering, Radiation
  • Transferrin / urine
  • alpha-Macroglobulins / urine


  • Transferrin
  • alpha-Macroglobulins